Abstract :
Aim:
To determine the effects of vitamin B6 supplement on serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST)levels and total glutathone in rats with nonalcoholic steatohepatitis (NASH).
Material and Method:
Male Sprague-Dawley rats were randomly divided into three groups.Group 1: fed ad libitum with regular dry rat chow for 6 weeks (control group,n =8).Group 2:fed ad libitum with 100%fat diet for 6 weeks to induce NASH (NASH group,n =8).Group 3:fed ad libitum with 100%fat and added 2 mg/kg/day of vitamin B6 orally (NASH+B6 group,n =10)for 6 weeks.All rats were sacrificed to collect blood at the end of the study.
Results:
Serum ALT and AST were significantly decreased in NASH group as compared with control (ALT;23.00 +1.92 U/L vs 40.13 + 2.35 U/L,AST;53.63 + 9.31 U/L vs 86.75 + 4.28 U/L,p <0.05),respectively. Total glutathione (GSH)was significantly increased in NASH group as compared with control group (2066.67 + 93.81 µM vs 1337.54 + 31.48 µM,p <0.05).Vitamin B6 treatment significantly increased serum ALT and AST level when compared to NASH group (ALT;76.30 + 6.21 U/L vs 23.00 + 1.92 U/L:AST;97.80 + 9.35 U/L vs 53.63 + 9.31 U//L p0.05),respectively.
Conclusion:
Vitamin B6 treatment involves in synthesis of aminotransferase that could response for the high serum ALT and AST and improves total GSH in rats with NASH. |
file/Thai-Journal-of-gastroenterology-vol-8-no-3-562284.pdf