Abstract :
Background: N-acetyl-p-aminophenol (APAP) or paracetamol overdose causes increasing of toxic me-
tabolites, which disrupting hepatocyte function, and liver injury occurs. Curcumin has been used for treatment of
inflammatory conditions such as hepatitis. Therefore, this study aims to determine effects of curcumin attenuate
hepatitis in mice with APAP overdose.
Methods: Male mice (25-30 gram) were divided into four groups. Group I (control); mice were gavaged
with distilled water. Group II (APAP); mice were gavaged with a single dose of 400 mg/kg of APAP. Group III
(APAP + CUR 200); mice were gavaged with a single dose of 400 mg/kg of APAP and 200 mg/kg of curcumin.
Group IV (APAP + CUR 600); mice were gavaged with a single dose of 400 mg/kg of APAP and 600 mg/kg of
curcumin. The serum was collected to determine liver enzymes and liver tissues for hepatic MDA, hepatic GSH
and histopathology.
Results: Serum ALT, AST and hepatic MDA were significantly increased in APAP when compared
with control and significantly decreased in APAP + CUR 200 and APAP + CUR 600 when compared with APAP.
Hepatic GSH was significantly decreased in APAP when compared with control and significantly increased in
APAP + CUR 200 and APAP + CUR 600 when compared with APAP. Histopathology of APAP showed acute
centrilobular hemorrhagic hepatic necrosis involving all zones and the improvement of liver pathology revealed in
APAP + CUR 200 and APAP + CUR 600.
Conclusion: These results suggest that APAP overdose is related to liver toxicity. Our results show
curcumin can prevent the damage by induction of hepatic GSH, reduction of oxidative stress, attenuation of liver
inflammation, and the improvement of liver pathology. In addition, curcumin at the dose of 600 mg/kg tends to be
more potent than 200 mg/kg. |
file/Thai-Journal-of-gastroenterology-vol-13-no-1-4517432.pdf