Abstract :
AIM: To examine the effects of Aloe vera on antioxidant level in rat with non-alcoholic steatohepatitis
(NASH) rats.
Materials and Methods: Rats were randomly divided into three groups. Group 1 (control group, n =
6), rats fed ad libitum with standard diet containing 35% of energy from fat, 47% from carbohydrate, and 18% from
protein for 8 weeks. Group 2 (NASH group, n = 6), rats fed ad libitum with high-fat high-fructose diet (HFHFD)
containing 55% of energy from fat, 35% from carbohydrate, and 10% from protein for 8 weeks. Group 3 (Aloe vera
group, n = 6), rats fed ad libitum with high-fat high-fructose diet (HFHFD) plus Aloe vera in DMSO (50 mg/kg) by
gavage feeding daily for 8 weeks. The concentration of Aloe vera was 50 mg/kg. All rats were sacrificed to collect
blood and liver samples at the end of treatment period.
Results: Hepatic malondialhyde (MDA) was increased significantly in the NASH group as compared
with the control group (MDA; 377 ± 77 vs. 129 ± 51 nmol/mg protein, p=0.000). The glutathione (GSH) in the
NASH group was also significantly decreased as compared with the control group (GSH; 9 ± 2 vs. 24 ± 8 nmol/mg
protein, p=0.001). The NASH group showed macrovesicular steatosis, microvesicular steatosis, cellular ballooning, and lobular
inflammation in microscopic study of paraffin section. On the other hand, the Aloe vera group showed significant
decrease in the levels of MDA compared with the NASH group (MDA; 199 ± 35 vs. 377±77 nmol/mg protein,
p=0.000), as well as improved GSH level (GSH; 18 ± 4 vs. 9 ± 2 nmol/mg protein, p=0.039). Aloe vera treatment
could alter MDA and GSH levels, and also improve the liver histopathology.
Conclusion: Aloe vera may attenuate the oxidative stress. It can increase GSH and improve liver histopathology
in rats with NASH.
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file/Thai-Journal-of-gastroenterology-vol-16-no-3-2878375.pdf