Abstract :
Background: Prevalence of NASH appears to be increasing and may lead to progressive hepatic fibrosis and eventually cirrhosis.
Aims: To develop a new high fat diet formula for rats and to establish a simplified and low cost in the NASH models.
Materials and Methods: Thirty male Sprague-Dawley rats weighing 220-250 gram were kept in Macrolon cages in a room temperature (25 0C) and humidity (55%), and a 12/12-hr light/dark cycle. The rats were divided into 4 groups; Group 1 : Feeding with 71% high-fat diet ; Group 2 : Feeding with 80% high-fat diet; Group 3 : Feeding with 100% high-fat diet; Group 4 (control) : Feeding with 35% fat standard diet. At the end of 3, 6, and 12 weeks, rats were sacrificed and livers were removed for grading of steatosis and necro-inflammation.
Results: Liver sections from rats fed 71% and 80% of fat diet showed mild steatosis at week 3, 6, and
12. There were no necro-inflammation revealed on histology in the rats fed with 71% and 80% of fat diet. There were moderate steatosis and mild lobular inflammation in 100% of fat diet-fed rats at week 3. There were moderate-severe steatosis and mild-moderate lobular inflammation in 100% of fat diet-fed rats at week 6. Moreover, the rats fed with 100% of fat at 12 weeks were found moderate steatosis, moderate inflammation and fibrosis with regeneration of hepatocytes in liver histology. Mallory body and focal perivenular necrosis was shown in 100% of fat diet-fed rats at week 6 and 12.
Conclusion: We were able to establish a simplified and reliable model of NASH with low cost in rats.
This model can be useful for future research study.
[Thai J Gastroenterol 2005; 6(3): 144-148] |
file/Thai-Journal-of-gastroenterology-vol-6-no-3-1493496.pdf